Part:BBa_K4811031:Design
PT7-TMS(Mn2)-TT7
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 164
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 122
Design Notes
As of 2023, the TMS is a novel BioBrick, and not much is known about the specifics of TMS to RBS binding, as well as how an aptamer insertion into the D-loop will alter this binding, both in presence and absence of the ligand.
Source
The part was ordered as a gBlock from IDT.
The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.
The manganese aptamer was adapted from Dambach, M., Sandoval, M., Updegrove, T. B., Anantharaman, V., Aravind, L., Waters, L. S., & Storz, G. (2015). The ubiquitous yybP-ykoY riboswitch is a manganese-responsive regulatory element. Molecular cell, 57(6), 1099–1109. https://doi.org/10.1016/j.molcel.2015.01.035